P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes
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authors
keywords
- P2X
- ATP-gated channels
- Double-electrode patch clamp
- Electrophysiology
- Receptor function
- Surface trafficking
- Xenopus
- Oocytes
document type
COUVabstract
Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.
Centre de Droit Social - UR 901
Aix Marseille Université | Tous droits réservés - Mentions légales